Efecto antibacteriano in vitro del extracto etanólico del zingiber officinale (kión) en cepas de escherichia coli
Date
2018-12-10
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Abstract
El objetivo del presente estudio fue determinar el efecto antibacteriano in vitro del extracto etanólico del Zingiber officinale “kión” en cepas de Escherichia coli. La muestra fue recolectada en la chacra de Nueva Florencia del distrito de Pichanaqui, provincia de Chanchamayo, ubicada en el departamento de Junín en el centro del Perú. La metodología de la investigación fue de tipo experimental y transversal. El extracto etanólico se obtuvo después de 5 días de maceración, luego se realizó el proceso de filtración; a la cual se le realizó la prueba de solubilidad, la marcha fitoquímica para determinar metabolitos secundarios. Para determinar el efecto antibacteriano, se aplicó el Método de Kirby Bauer (Difusión en Agar). Las concentraciones aplicadas del extracto etanólico del Zingiber officinale “kión” fueron de 25%, 50% y 100%, cuyos resultados mostraron un halo de inhibición de 10mm, 6 mm y 6 mm respectivamente, se comparó con controles de Gentamicina 10 µg y etanol. Se llegó a la conclusión que el extracto etanólico del Zingiber officinale “kión” a concentración del 25% presentó efecto antibacteriano en cepas de Escherichia coli
The objective of the present study was to determine the in vitro antibacterial effect of the ethanolic extract of Zingiber officinale "kión" in strains of Escherichia coli. The sample was collected in the Nueva Florencia farm in the district of Pichanaqui, province of Chanchamayo, located in the department of Junín in central Peru. The methodology of the research was experimental and transversal. The ethanolic extract was obtained after 5 days of maceration, then the filtration process was carried out; to which the solubility test was carried out, the phytochemical march to determine secondary metabolites. To determine the antibacterial effect, the Kirby Bauer method (Agar diffusion) was applied. The applied concentrations of the ethanolic extract of Zingiber officinale "kión" were of 25%, 50% and 100%, whose results showed a halo of inhibition of 10mm, 6 mm and 6 mm respectively, it was compared with controls of Gentamicin 10 μg and ethanol. It was concluded that the ethanolic extract of Zingiber officinale "kión" at a concentration of 25% presented an antibacterial effect in strains of Escherichia coli
The objective of the present study was to determine the in vitro antibacterial effect of the ethanolic extract of Zingiber officinale "kión" in strains of Escherichia coli. The sample was collected in the Nueva Florencia farm in the district of Pichanaqui, province of Chanchamayo, located in the department of Junín in central Peru. The methodology of the research was experimental and transversal. The ethanolic extract was obtained after 5 days of maceration, then the filtration process was carried out; to which the solubility test was carried out, the phytochemical march to determine secondary metabolites. To determine the antibacterial effect, the Kirby Bauer method (Agar diffusion) was applied. The applied concentrations of the ethanolic extract of Zingiber officinale "kión" were of 25%, 50% and 100%, whose results showed a halo of inhibition of 10mm, 6 mm and 6 mm respectively, it was compared with controls of Gentamicin 10 μg and ethanol. It was concluded that the ethanolic extract of Zingiber officinale "kión" at a concentration of 25% presented an antibacterial effect in strains of Escherichia coli
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Keywords
Zingiber officinale “kión”, Escherichia coli, Método kirby bauer, Halo de inhibición, Concentración, Efecto antibacteriano, Zingiber officinale "kión", Escherichia coli, Kirby bauer method, Inhibition halo, Concentration, Antibacterial effect