Actividad antibacteriana del extracto acuoso solanum tuberosum (papa fermentada) y aceite esencial thymus vulgaris (tomillo), frente a cepa staphylococcus aureus, estudio in vitro
Date
2017-11-14
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Abstract
El presente estudio ha sido realizado con el objeto de determinar la actividad antibacteriana in vitro del extracto acuoso de Solanum tuberosum (papa fermentada) y del aceite esencial Thymus vulgaris (tomillo), frente a cepa Staphylococcus aureus. Método experimental in vitro. Se efectuó en tres etapas: la primera etapa, que consistió en un estudio piloto, la segunda etapa, correspondió a la realización de la marcha fitoquímica para ambas muestras y, la tercera, al sembrado con cepa liofilizada de Staphylococcus aureus ATCC® 29213TM en agar Müller-Hinton, utilizando la técnica de difusión en agar con discos impregnados. Se trabajaron 5 placas con discos de cefalexina 30μg y del extracto acuoso al 5 por ciento de Solanum tuberosum (chuño negro), 5 placas con aceite esencial de Thymus vulgaris (tomillo) al 90 por ciento. Los resultados de la marcha fitoquímica del extracto acuoso del Solanum tuberosum (chuño negro) presenta aminoácidos, alcaloides y flavonoides (antocianinas). En el aceite esencial del Thymus vulgaris se presentan terpenoides, taninos y compuestos fenólicos. Los resultados del halo de inhibición del extracto acuoso del Solanum tuberosum (chuño negro) 20,8 ± 0.3mm, Thymus vulgaris (tomillo) 15.7 ± 0.3mm y cefalexina 31.2 ± 0.3mm. Se concluye que frente a cepa Staphylococcus aureus el extracto acuoso de Solanum tuberosum (chuño negro) tiene actividad antibacteriana considerada como sensible según CLSI y sumamente sensible para Duraffourd, y en el caso del aceite esencial Thymus vulgaris (tomillo), actividad antibacteriana intermedia, según CSLI, y muy sensible para Duraffourd.
The present study has been carried out in order to determine the in vitro antibacterial activity of the aqueous extract of Solanum tuberosum (fermented potato) and of the essential oil Thymus vulgaris (thyme), against the Staphylococcus aureus strain. In vitro experimental method. It was carried out in three stages: the first stage, which consisted of a pilot study, the second stage, corresponded to the performance of the phytochemical march for both samples and, the third, to the sowing with lyophilized strain of Staphylococcus aureus ATCC® 29213TM in agar Müller-Hinton, using the agar diffusion technique with impregnated discs. Five plates with cephalexin discs 30μg and 5 por ciento aqueous extract of Solanum tuberosum (black chuño), 5 plates with essential oil of Thymus vulgaris (thyme) 90 por ciento were worked. The results of the phytochemical march of the aqueous extract of Solanum tuberosum (black chuño) present amino acids, alkaloids and flavonoids (anthocyanins). In the essential oil of Thymus vulgaris terpenoids, tannins and phenolic compounds occur. The results of the inhibition halo of the aqueous extract of Solanum tuberosum (black chuño) 20.8 ± 0.3mm, Thymus vulgaris (thyme) 15.7 ± 0.3mm and cephalexin 31.2 ± 0.3mm. It is concluded that against a Staphylococcus aureus strain the aqueous extract of Solanum tuberosum (black chuño) has antibacterial activity considered as sensitive according to CLSI and extremely sensitive for Duraffourd, and in the case of the essential oil Thymus vulgaris (thyme), intermediate antibacterial activity, according to CSLI, and very sensitive for Duraffourd.
The present study has been carried out in order to determine the in vitro antibacterial activity of the aqueous extract of Solanum tuberosum (fermented potato) and of the essential oil Thymus vulgaris (thyme), against the Staphylococcus aureus strain. In vitro experimental method. It was carried out in three stages: the first stage, which consisted of a pilot study, the second stage, corresponded to the performance of the phytochemical march for both samples and, the third, to the sowing with lyophilized strain of Staphylococcus aureus ATCC® 29213TM in agar Müller-Hinton, using the agar diffusion technique with impregnated discs. Five plates with cephalexin discs 30μg and 5 por ciento aqueous extract of Solanum tuberosum (black chuño), 5 plates with essential oil of Thymus vulgaris (thyme) 90 por ciento were worked. The results of the phytochemical march of the aqueous extract of Solanum tuberosum (black chuño) present amino acids, alkaloids and flavonoids (anthocyanins). In the essential oil of Thymus vulgaris terpenoids, tannins and phenolic compounds occur. The results of the inhibition halo of the aqueous extract of Solanum tuberosum (black chuño) 20.8 ± 0.3mm, Thymus vulgaris (thyme) 15.7 ± 0.3mm and cephalexin 31.2 ± 0.3mm. It is concluded that against a Staphylococcus aureus strain the aqueous extract of Solanum tuberosum (black chuño) has antibacterial activity considered as sensitive according to CLSI and extremely sensitive for Duraffourd, and in the case of the essential oil Thymus vulgaris (thyme), intermediate antibacterial activity, according to CSLI, and very sensitive for Duraffourd.
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Keywords
Aceite esencial, Actividad antibacteriana, Extracto acuoso, Papa fermentada, Solanum tuberosum, Thymus vulgaris, Staphylococcus aureus, Essential oil, Antibacterial activity, Aqueous extract, Fermented potato, Solanum tuberosum, Thymus vulgaris, Staphylococcus aureus