Aislamiento e identificación de algunos metabolitos secundarios presentes en el extracto etanólico de las hojas de tagetes multiflora H.B.K. “Chikchimpa”
Date
2017-07-05
Journal Title
Journal ISSN
Volume Title
Publisher
Universidad Inca Garcilaso de la Vega
Abstract
El presente trabajo tuvo por finalidad determinar los componentes presentes en el extracto etanólico de las hojas de Tagetes multiflora H.B.K. recolectado en el departamento de Puno, provincia de Lampa, distrito de Pucará, se obtuvo una maceración etanólica que se concentró por evaporación a estufa a 40°C hasta obtener un extracto seco y con éste se procedió a realizar una marcha de solubilidad y tamizaje fitoquímico; se realizó el análisis cromatográfico, mediante cromatografía en capa fina analítica, luego cromatografía en columna rápida para fraccionar en subextractos tal como: diclorometano, etanol y agua, comprobándose mediante cromatografía en capa fina analítica que el subextracto etanólico presenta mayor cantidad de metabolitos secundarios detectados mediante el screening fitoquímico, por lo que se procedió a realizar cromatografía en capa fina a escala preparativa, logrando aislar 4 componentes fenólicos, identificando su estructura química mediante espectroscopia UV-Vis, encontrándose derivados de flavonas que fueron confirmados mediante reacciones de desplazamiento en espectroscopia UV-Vis
The aim of this study was to determine the components of Tagetes multiflora H.B.K. leaves’ ethanolic extract, collected at Puno department, Lampa province, Pucará district, which was obtained by ethanol maceration, followed by evaporation at 40°C, obtaining a dry extract, followed by a solubility march and phytochemical screening; the chromatographic analysis was performed by analytical thin layer chromatography, followed by flash chromatography to fractionate the extract in sub-extracts such as dichloromethane, ethanol and water. Through analytical TLC, it was find that the ethanolic sub-extracts showed more secondary metabolites which were detected by phytochemical screening. Consequently, a preparative scale TLC was performed, isolating four phenolic compounds, which chemical structure was identified by UV- Visible Spectroscopy, obtaining flavone derivatives, which presence was later confirmed by UV- Visible Spectroscopy displacement reactions
The aim of this study was to determine the components of Tagetes multiflora H.B.K. leaves’ ethanolic extract, collected at Puno department, Lampa province, Pucará district, which was obtained by ethanol maceration, followed by evaporation at 40°C, obtaining a dry extract, followed by a solubility march and phytochemical screening; the chromatographic analysis was performed by analytical thin layer chromatography, followed by flash chromatography to fractionate the extract in sub-extracts such as dichloromethane, ethanol and water. Through analytical TLC, it was find that the ethanolic sub-extracts showed more secondary metabolites which were detected by phytochemical screening. Consequently, a preparative scale TLC was performed, isolating four phenolic compounds, which chemical structure was identified by UV- Visible Spectroscopy, obtaining flavone derivatives, which presence was later confirmed by UV- Visible Spectroscopy displacement reactions
Description
Keywords
Tagetes multiflora H.B.K., Flavona, Espectroscopia UV-Vis, Reacciones de desplazamiento, Tagetes multiflora H.B.K., Flavone, UV-Vis spectroscopy, Displacement reactions